市場(chǎng)上有很多名義上是總RNA提取的試劑盒,但是實(shí)際上很難提取到全部的RNA,特別是小分子RNA基本上無(wú)法提取。這對(duì)于研究者來(lái)說(shuō)是非常大的損失。小編今天給大家介紹一些Norgen Biotek的總RNA提取試劑盒,可以完整的提取各種類型的RNA:mRNA,lncRNA ,small RNA,microRNA (miRNA) 等。
Norgen Biotek 總RNA(含microRNA)提取試劑盒#NGB-17200提取后的總RNA可直接用于下游應(yīng)用:qRT-PCR, Northern blots, microarrays, NGS, miRNA profiling, biomarker discovery, Primer extension, RNase protection, pathogen detection等.
總RNA(含microRNA)提取試劑盒原理:基于使用Norgen專利樹(shù)脂作為分離基質(zhì)的離心柱色譜。
總RNA(含microRNA)提取試劑盒特點(diǎn):
1、提取高質(zhì)量和純度的總RNA,包括miRNA。
2、磚利配方,試劑盒不含苯酚或氯仿步驟。(它們會(huì)抑制偶聯(lián)標(biāo)記處理,以及PCR擴(kuò)增,同時(shí)對(duì)GC含量有偏好)。
3、結(jié)合并洗脫所有的RNA,不論其大小或GC含量如何。
4、無(wú)論GC含量如何,均可高效提取小RNA。
5、提取效果非常敏感和線性,而不需要載體RNA。
6、方便快捷的離心柱操作方式。
7、適用樣品類型全面:細(xì)胞,組織,細(xì)菌,酵母,血清/血漿,唾液,腦脊髓液等等。
MicroRNA芯片檢測(cè)microRNA提取的豐度:
總RNA(含microRNA)提取試劑盒樣品使用量與RNA產(chǎn)出數(shù)據(jù)參考:
最大樣品使用量 RNA提取產(chǎn)量
Liver (10 mg) 30 μg
Kidney (10 mg) 10 μg
Brain (10 mg) 12 μg
Blood (hamster, 100μL) 5 μg
HeLa (1 x 106) 15 μg
CHO (1 x 106) 11 μg
Yeast (1 x 108) 30 μg
E. coli (1 x 109) 43 μg
《Nature & Cell高分發(fā)表文章:總RNA提取試劑盒》
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